Dr. Moo-Yeal Lee, Associate Professor of Biomedical Engineering Department at University of North Texas will give a seminar titled "Pillar and Perfusion Platform for Dynamic Human Organoid Culture and Analysis" to the interested faculty and students at Discovery Park.

Abstract

Several three-dimensional (3D) cell culture platforms have been developed, including ultra-low attachment well plates, Transwell inserts, hanging droplet plates, and microfluidic devices. However, these platforms have relatively low throughput and/or are unsuitable for high-throughput organoid culture and analysis in situ. To facilitate dynamic organoid culture in a high-throughput screening (HTS) system, we have developed a pillar plate and a complementary perfusion plate along with "microarray 3D bioprinting" technology, which is highly flexible, user-friendly, and easily combined with conventional 384-well plates to support organotypic cell cultures and multiplexed high-content imaging assays. We have successfully demonstrated reproducible and scale-up production of human liver organoids in the pillar/perfusion plate for predictive hepatotoxicity screening.

Biography

Dr. Moo-Yeal Lee (bioprinting.engineering.unt.edu) is an associate professor in the department of biomedical engineering at UNT and the founder and president of Bioprinting Laboratories Inc. (3dbpl.com) Dr. Lee received his Ph.D. degree from KAIST, South Korea, and worked at Tokyo Institute of Technology as a postdoctoral fellow supported by Japan Society for the Promotion of Science (JSPS). He moved to the US in 2001 and worked at Rensselaer Polytechnic Institute, Solidus Biosciences Inc., and Cleveland State University before joining UNT in 2021. Dr. Lee has been awarded multiple grants from the NIH and NSF for his innovative research in developing 3D bioprinting technology and associated pillar/perfusion plate platforms for high-throughput, predictive compound screening. He has published 86 research articles in peer-reviewed journals, including prestigious Nature Communications, PNAS, and Biofabrication, 19 patents and patent applications, 5 book chapters, and 1 book.